> I used follow shift changes which works very nice now. I didn't worry about
> error analysis just yet, my problem is more trivial: When I click on Show
> Peaks in the follow window I get an empty peak list and the following error
> appears:
>
> Exception in Tkinter callback
> Traceback (most recent call last):
> File "/usr/local/ccpnmr/python2.4/lib/python2.4/lib-tk/Tkinter.py", line
> 1345, in __call__
> return self.func(*args)
> File
> "/usr/local/ccpnmr/ccpnmr1.0/python/ccpnmr/analysis/FollowShiftChangesPopup.py",
> line 942, in showPeaks
> self.guiParent.viewPeaks(peaks)
> File
> "/usr/local/ccpnmr/ccpnmr1.0/python/ccpnmr/analysis/AnalysisPopup.py", line
> 1158, in viewPeaks
> popup.update(peaks)
> File
> "/usr/local/ccpnmr/ccpnmr1.0/python/ccpnmr/analysis/SelectedPeaksPopup.py",
> line 663, in update
> if peak.isDeleted:
> AttributeError: 'float' object has no attribute 'isDeleted'
>
>
> when I click on Show peak in the fit graph window nothing at all happens,
> not even an error message. I am sure this feature was working before, so
> maybe a side effect of the recent upgrades?
Hi Mark,
Unfortunately I cannot reproduce this, and the line number in your error
trace does not match the current version of the FollowShiftChanges popup.
Maybe you are missing some of the newest updates given the recent
kerfuffle?
(The disfunctional Show Peak from the graph would be a consequence of the
first issue)
> Also: I am sure that the "group peaks" previously also had the option to
> pick peaks in an experiment series based on the first being picked but that
> option appears to have disappeared. Is it possible to get it back?
This is present in the Rates Analysis option, but was never present in the
Follow Shift Changes.
> Finally, I get really nice fits with the A(B+x-sqrt... function for a
> binding curve but would it be possible to explain if A and B have a physical
> meaning? Maybe I have to be patient until the manual is done but it would be
> nice to know a bit more (help is empty).
As far as I recall this function is used to derive binding constants.
Unless Wayne knows offhand, we'll look up the derivation for you.
T.
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Dr Tim Stevens Email: [log in to unmask]
Department of Biochemistry [log in to unmask]
University of Cambridge Phone: +44 1223 766018 (office)
80 Tennis Court Road +44 7816 338275 (mobile)
Old Addenbrooke's Site +44 1223 364613 (home)
Cambridge CB2 1GA WWWeb: http://www.bio.cam.ac.uk/~tjs23
United Kingdom http://www.pantonia.co.uk
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