Hi Kaare,
Could you send me a screen shot of the two spectra, focussing on a copied
peak pair and include a view if the edit assignment popup? I'm a bit
baffled, but seeing things may help.
Thanks,
T.
> >> What is not so fine is that only the assignment in the carbon dimension
> >> is copied. The assignment in the hydrogen dimension is lost.
> >>
> >> Is 'reproduce peaks' supposed to behave like this?
> >
> >No, and it doesn't do this when I test. One thing that springs to mind is
> >that the two spectra may be under two different shift lists and you
> >already have 1H shifts at positions different to the destination spectrum,
> >thus the 1H resonances are outside of the assignment tolerances and the
> >assignment is not transferred. - If this is the case you could use a
> >completely new shift list or the same one as the source via the Shift List
> >column in Menu::Experiment::Edit Experiments.
>
> I have set up the project so that I have one 'Experiment' with all my HSQC
> with the different decoupling powers that I want to calibrate. For each HSQC
> I then have two peak-lists, one for the left and one for the right part of
> each doublet.
>
> So here is what I did:
> First I added peaks in my left and right lists in an undecoupled spectrum.
> Then I opened a new HSQC (with cw-decoupling on) in the same experiment as
> the undecoupled spectrum. In this spectrum the 1H shifts has changed for the
> left and right parts of the doublets.
> If I now clone the peaks from the undecoupled spectrum to the empty peak
> list of the decoupled spectrum a get a copy of the peak a the expected
> position (where there is no intensity) with assignment in both the H and C
> dimensions.
> If I instead reproduce the peaks only the assigenment in the C dimension is
> copied. The peaks are positioned at the same coordinates as in the source
> spectrum.
>
>
> >If this is not the case, maybe have a look if the appropriate 1H resonance
> >options are present in the Edit Assignment popup for a copied peak, and if
> >you can manually set the correct assignment there. This will give me a
> >better idea of the situation.
>
> If I use the Edit Assignment popup on the reproduced peaks it does suggest
> the right assignment.
>
> Kaare
>
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Dr Tim Stevens Email: [log in to unmask]
Department of Biochemistry [log in to unmask]
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