Sure, here they are.
There are three copies in ASU, all have more or less similar
density, blue contour at 1.0sigma for 2fo-fc and magenta
at 2.5sigma for fo-fc. The shown residues are the second
valines which fit well.
Thanks.
Jie
[log in to unmask] wrote:
> Can you show the map?
>
> Anthony
>
> > Dear all
> >
> > I am refining a peptide structure with an N-terminal throenine.
> > However, the density map of the N-terminal part looks strange,
> > the first throenine does not fit in well while the second valine
> > fits very well. It seems that this throenine was modified somehow
> > during protein production. The peptide was expressed in E.Coli
> > and cleavaged by Formic Acid and Cyanogen Bromide, then
> > purified by HPLC and lyophilized.
> >
> > Does anyone have similar experience of a modified N-terminal
> > throenine? If so, please kindly point to me a reference.
> >
> > Thank you.
> >
> > Jie Liu
> >
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