Can you show the map?
Anthony
> Dear all
>
> I am refining a peptide structure with an N-terminal throenine.
> However, the density map of the N-terminal part looks strange,
> the first throenine does not fit in well while the second valine
> fits very well. It seems that this throenine was modified somehow
> during protein production. The peptide was expressed in E.Coli
> and cleavaged by Formic Acid and Cyanogen Bromide, then
> purified by HPLC and lyophilized.
>
> Does anyone have similar experience of a modified N-terminal
> throenine? If so, please kindly point to me a reference.
>
> Thank you.
>
> Jie Liu
>
|