Dear All,
I have been trying to get a protein-ligand complex crystal for a long
time. Here, ligand is either substrate or product for this kinase. I
have tried many methods: soaking with apo-crystal, co-crystal with
different concentration of ligand and screen for new conditions. I got
a couple of co-crystals with the new conditions, but still no ligand
was found in the active site.
Anybody has some new ideas that I can try? I have been using glycerol
as my cryoprotectant. Could cryoprotectant have some negative effect
on co-crystal? I will try to collect some data at room temperature in
the future.
Thank you in advance for your suggestion.
Nian
Dept of Biochemistry
UT Southwestern Medical Center
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