In Coot - turn on the "Cell & Symmetry..." option (in the Draw menu) for
your molecule, in order to visualise the other
molecules within your crystal.
Then use "Save Symmetry Coordinates..." (in the File menu).
Click on the isolated bit of your structure that is closest to the rest
of your molecule (and/or the one that makes the most
sense sequence-wise / biologically).
Save this as a pdb-file.
Do a bit of cut-and-paste to put the transformed coordinates into your
main pdb file.
Bob is then quite possibly your uncle.
Regards,
Tony.
bc_panlf wrote:
>> Hi all:
>> I am a beginner for protein crystallography. After I used Arp/wrap to
>> build a model structure from molecular replacement, one part of the output
>> model structure is very strange, the first beta strand is isolated and not
>> fit to the other part. someone tell me coot can do this ,somehow
>> symmetrically translate. but i don't know how.can anyone tell me how to
>> fix this problem?
>> i post the pdb and mtz file in the attachment.
>> Thank you very much !!
>>
>>
--
Dr Antony W Oliver
CR-UK DNA Repair Enzymes Group
Section of Structural Biology
The Institute of Cancer Research
237 Fulham Road
Chelsea
London SW3 6JB
Phone: 020 7153 5451 / 5571
Fax: 020 7153 5457
E-mail: [log in to unmask]
--
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