Dear all,
what does it mean when a structure doesn't have many water molecules?
I have a 2.3A data set, 2 molecules in the AU (260 residues each), space
group C2221 - I haven't finished my water search but I don't seem to have
more than 20-30 obvious/good water molecules. The Rfac/Rfree are 22/30%,
the protein is modeled and the ligands are in.
I collected the data at APS SERCAT ID-22. Did I have a dry crystal (!!) or
did the beamline dried it up?!
How many water molecules should I expect at this resolution?
Also, what does it mean when a strong peak in the Fobs-Fc doesn't have the
corresponding 2Fobs-Fc map covering it even at very low sigma?
Thank you!
Eli :o)
PS: I have attached the statistics for the data set from XDS
--
Elisabetta Sabini, Ph.D.
Research Assistant Professor
University of Illinois at Chicago
Department of Biochemistry and Molecular Genetics
Molecular Biology Research Building, Rm. 1108
900 South Ashland Avenue
Chicago, IL 60607
U.S.A.
Tel: (312) 996-6299
Fax: (312) 355-4535
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