Rob,
A couple of years ago my colleague and I "mined" the crystallization
data in REMARK 280 from 3939 entries in the PDB. In 109 entries,
glycerol came out as the ingredient with the highest concentration
that was measured with a % (percent) sign i.e. including '%', '%W/V',
'%V/V' etc.
The average concentration of these 109 entries was 15.5%
Many of these appeared to be "salting in" conditions.
So 10% glycerol should be no problem - it may be close to the overall
average (which we don't know).
Patrick
On 8/25/07, Rob Gruninger <[log in to unmask]> wrote:
>
>
>
> Dear All,
> I am working with a protein that requires 10% glycerol throughout the
> purification to keep it soluble. I have been very worried that having
> glycerol in my protein solution when I am trying to crystallize it will
> prevent me from obtaining crystals. I am curious to see if others have
> successfully obtained crystals of proteins that required glycerol to keep
> them soluble and if so what concentration of glycerol could you use?
> Should I be concerned about this or am I worrying too much?
> Thanks for your input
> Rob
>
> --
> Robert J.Gruninger B.Sc.
> PhD Student
> Department of Chemistry & Biochemistry
> University of Lethbridge, 4401 University Dr.
> Lethbridge, AB, Canada, T1K 3M4
> Phone:(403)329-2746
> Fax:(403)329-2057
>
|