There are four molecules in the asymmetric unit. And this density is
present in every molecules.
-----Original Message-----
From: ANA MARIA MISIC [mailto:[log in to unmask]]
Sent: Thursday, July 26, 2007 5:24 PM
To: Wu, Mousheng
Subject: Re: [ccp4bb] unknown density in structure
Hi Mousheng - Did you check to see if that falls on a special position?
That can lead to a buildup of electron density, and the symmetrical
shape is suspect.
good luck
Ana M. Misic
Research Assistant
Department of Biomolecular Chemistry
Department of Bacteriology
University of Wisconsin - Madison
420 Henry Mall, Rm 230
Madison, WI 53706
Lab Phone: (608)265-9282
Fax: (608)262-9865
----- Original Message -----
From: "Wu, Mousheng" <[log in to unmask]>
Date: Thursday, July 26, 2007 4:21 pm
Subject: [ccp4bb] unknown density in structure
To: [log in to unmask]
> Hi, everyone,
>
>
>
> I am recently solving a structure at 1.8A. After I fitted all residues
> into the model, I found that there was an extra density in a pocket
> consisted of E, N, R and other hydrophobic residues. The density is
> strong and connected like a butterfly. I checked the crystallization
> condition. I can not figure out what it is. It seems that it is not
from
> the crystallization components.
>
>
>
> Does anyone know how to figure out what the density is? Is there any
> program or database I can use to predict the potential ligand?
>
> Thanks.
>
>
>
> Mousheng
>
|