1) Combine your MAD 3A phases with the 1.7A native data and use DM or
Pirate to refine and extend the phase set.
2) Make sure your MR solution is on the same origin as the MAD phases.
You can calculate PHIC and FOM from MR solution, combinre with the MAD
phases and do an anomalous diference map, then use the sites you find to
do the MAD phasing.
Or combine the PHIC/FOM, and the MAD phases into one mtz file and run
the Clipper utility for phase comparison. That moves the phases so they
are consistent .
Then you can run REFMAC using the MAD extended phases as restraints -
you get out map coefficients with combined phase information which
should be easy to bulid the rest of your structure into
Eleanor
Joe Batchelor wrote:
> Hi,
>
> I have a 1.7 A native dataset, a good MR solution for 2/3 of the
> protein, and MAD phases to 3 A. How should I combine the MR phases
> with the MAD phases?
>
> Thanks,
> Joe
>
>
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