>If you want to combine though, I use the molrep phases to get all
>heavy atoms
>in an anomalous fourier map, then go to SHARP with these sites and
>use the 'External phases' option
>in the SHARP gui. Works well for me.
This has worked well for me too...I highly recommend SHARP.
Good luck-
Steve
-----Original Message-----
From: CCP4 bulletin board [mailto:[log in to unmask]] On Behalf Of
Anastassis Perrakis
Sent: Tuesday, July 17, 2007 12:56 PM
To: [log in to unmask]
Subject: Re: [ccp4bb] Combining MR and MAD phases
I would be tempted to say that you don't need to combine; with 1.7 A
data, refinement should do it with no trouble.
And, yes, of course ARP/wARP (7.0) would be the best way to do it ;-)
If you want to combine though, I use the molrep phases to get all
heavy atoms
in an anomalous fourier map, then go to SHARP with these sites and
use the 'External phases' option
in the SHARP gui. Works well for me.
I also recall that Phaser has an option for exactly do that, but with
SAD data only.
But, again, if you have 1.7 A data good old refinement and auto-
building will (likely) do the job in a glance.
Tassos
PS If you dont want to install arp/warp locally why not try:
http://cluster.embl-hamburg.de/ARPwARP/remote-http.html
PS2 Apologies for the shameless promotion of ARP/wARP ;-)
On 17 Jul 2007, at 18:22, Joe Batchelor wrote:
> Hi,
>
> I have a 1.7 A native dataset, a good MR solution for 2/3 of the
> protein, and MAD phases to 3 A. How should I combine the MR phases
> with the MAD phases?
>
> Thanks,
> Joe
------------------------------------------------------------------------------
Notice: This e-mail message, together with any attachments, contains
information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station,
New Jersey, USA 08889), and/or its affiliates (which may be known
outside the United States as Merck Frosst, Merck Sharp & Dohme or MSD
and in Japan, as Banyu - direct contact information for affiliates is
available at http://www.merck.com/contact/contacts.html) that may be
confidential, proprietary copyrighted and/or legally privileged. It is
intended solely for the use of the individual or entity named on this
message. If you are not the intended recipient, and have received this
message in error, please notify us immediately by reply e-mail and then
delete it from your system.
------------------------------------------------------------------------------
|