2007/7/12, Gebhard Schertler <[log in to unmask]>:
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> I have obtained some promising X-tals in 2M Ammonium Nitrate. I am now looking for freezing
> conditions. Has anybody had experience in successfully freezing X-tals under similar conditions
> ? What was the best cryo-protectant and which concentration was optimal ?
Hi,
You have some informations in supplementary methods of this ref:
Structural insight into antibiotic fosfomycin biosynthesis by a
mononuclear iron enzyme
Luke J. Higgins, Feng Yan, Pinghua Liu, Hung-wen Liu and Catherine L. Drennan
Nature 437, 838-844 (1 October 2005)
Informations are in the first doc at this page:
http://www.nature.com/nature/journal/v437/n7060/suppinfo/nature03924.html
30% xylitol, 2.0M ammonium sulfate, and 100mM Tris-HCl pH 8.0
Some other good cryoprotectants are small PEGs, glycerol, MPD...
Michel.
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