Gary S. Thompson wrote:
> Wayne Boucher wrote:
>
>> Hello,
>>
>> I think Tim will have to be the one who answers this properly (he's back
>> on Thursday). But it looks to me like the "Strip locations" is a
>> generalisation of the "Make Strips" (so I'm not quite sure why the
>> latter
>> has to exist), in particular you can do various combinations of what
>> peak dimensions are being mapped to what window dimensions.
>>
>> And it depends what you mean by "centre the shift". It is supposed to
>> take the centre for the x and z axes for each strip, but obviously
>> this is
>> not possible for the y, where instead it takes an average of all the
>> strips. If this is not happening for you then I would recommend hitting
>> the button a second time just in case (I seem to remember sometimes it
>> didn't feed through correctly first time around) and also check if there
>> are any exceptions in the console. (I just tried mucking around here
>> and
>> it seemed to work ok.)
>>
>> Wayne
>>
>>
> Dear All
>
> here is some followup that may help and clarify my earlier problem
>
> specifically what I was expecting was that when I select peaks for say
> each amide 2-10 in an hsqc peaks list and do strip locations on a 3d
> I should be able to see the chmical shift of each amide I have
> selected in its respective strip and it should be centerered (this
> ought occur surely as the whole joy of strips is that you know where
> the peaks should be...) Currently I do this and analysis seems to
> take me to the right plane (e.g. for a 1H 15N hsqc and a 1H-15N hsqc
> noesy [x 1H amide y 1H noesy z 15N amide] I get to the correct 15N
> plane but the 1H shift for the amide on the x axis is typically well
> outside the strip)
>
> as an additional commment it would be nice to be able to use strip
> locations without changing the shift in the y dimension of the target
> window. e.g. if you are looking at strips in an hsqc noesy and want to
> coencettrate on methyls moving the y dimension back down to the amides
> each time is a pain
>
> regards
> gary
>
Ok here is some more followup. The problem seems to be that the spectrum
panels are not centering themselves on the correct x shifts sometimes so
I have either hit strip locations twice or goto position...
regards
gary
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Dr Gary Thompson
Astbury Centre for Structural Molecular Biology,
University of Leeds, Astbury Building,
Leeds, LS2 9JT, West-Yorkshire, UK Tel. +44-113-3433024
email: [log in to unmask] Fax +44-113-2331407
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