Quoting Priyank Maindola <[log in to unmask]>:
> Hello all,
> I am struggling with getting the phases out for my protein. Heavyatom
> database shows that only mercury and samarium have the binding motifs in
> it.
>
> Sodium iodide soak is killing the crystal even as less as 0.2 Molar
> concentration for 30sec.
> With Potassium iodide crystal is okay upto 0.75M for 1 & a half min but
> the
> incorporation is not at relevent sites.(Iodide signal is not distinct and
>
> is weak)
how much data and what is anomalous redundancy? what space group?
could you do Sulphur-SAD?
> Hg & Au salts precipitate it.
i dont understand this, precipitate the crystals?
what concentrations and soak times do you use and what is the pH?
...ie more details would be useful.
> With Pt signal is weak.
again how about a longer soak??? higher concentration???
... or try Pd, should be more reactive.
> Somewhere I read that high salt causes problems for heavy atom to bind to
>
> protein.My protein needs high salt for stability (750mM NaCl)
I think that would mainyl affect electrostatic binding, but imidazole
of cys attachement of Hg, Pr etc probably not. (??????)
and again, why no se-met??????
> Please help with some suggestion!!
>
tommi
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