Hi,
give Ta6Br12 a try - it is a cluster with an incredible white line -
really nice for phasing :-). But the best thing is that it does not mind
high ionic strength (worked great for me). There are a couple of
references out for it which you can check for more details...
Good luck, Petra
> Hello all,
> I am struggling with getting the phases out for my protein. Heavyatom
> database shows that only mercury and samarium have the binding motifs in
> it.
>
> Sodium iodide soak is killing the crystal even as less as 0.2 Molar
> concentration for 30sec.
> With Potassium iodide crystal is okay upto 0.75M for 1 & a half min but
> the
> incorporation is not at relevent sites.(Iodide signal is not distinct and
> is weak)
> Hg & Au salts precipitate it.
> With Pt signal is weak.
> Somewhere I read that high salt causes problems for heavy atom to bind to
> protein.My protein needs high salt for stability (750mM NaCl)
>
> Please help with some suggestion!!
>
--
Petra Verdino, PhD
Research Associate
The Scripps Research Institute, BCC206
10550 North Torrey Pines Road
CA 92037 La Jolla, USA
tel:1-858-784-2294
fax:1-858-784-2980
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