Hello all,
I am pretty new to macromolecular NMR in general and the
CCPNMR programs in particular, so bear with me :-) . I think
I am also seeing the spectrum duplication issue.
I am working on some triple-resonance spectra of a small
protein, and I am looking at (among others) a HNCACB and
and a HNCO spectrum in a 3D window with 1H, 13C and 15N
axes. The HNCACB spectrum has 13C frequencies in the
range from 8 to 88 ppm, while the HNCO spectrum ranges
from 165 to 185 ppm.
While picking and assigning peaks, a second copy of the
HNCACB spectrum appeared, with the frequency range shifted
to 88-165 pm (fitting snugly between the proper limits
of the two spectra). Some time later, six equally-spaced
"ghost images" of the HNCO spectrum appeared, with the
top one reaching up to 50pm on the 13C axis.
(Image at http://ultr23.vub.ac.be/~lieven/spectra.png )
--
Lieven Buts
Ultrastructure Laboratory
Vrije Universiteit Brussel
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