On Thu, 14 Jun 2007, Robert Dagil wrote:
> {1}[H-x][C-x]{1}[N-x]
>
> Is there a way to link the F2 assignment to the same residue as the F1 and
> F3, giving a tag looking like this:
>
> {1}[H-x]{1}[C-x]{1}[N-x]
>
> And wouldn't this be a more "correct" tag?
The idea is that you will map a spin system to a specific residue so for
the hncoca the ca should not be from the same spin system. When you link
spin systems sequentially you will merge this resonance into the spin
system corresponding to the residue preceeding the spin system of the H &
N.
> The other issue I have is assigning the hnca spectrum. I want to copy the
> peaks already picked from the hncoca spectrum to my hnca spectrum, and I've
> tried using the "copy peak assignments" function without success - is there
> a way to do this?
The philosophy is that it is more 'proper' to genuinely pick peaks except
where there are special circumstances (overlap, etc.), so you should
ideally do just as you have done with the hncoca. The slight pain is that
if you use the automatic assignment of the non-root resonances, you will
now have two resonances for the ca(i-1), where the peak appears. At some
point you will want to merge these resonances as they represent the same
atom.
--
Dr. Brian O. Smith ---------------------- B Smith at bio gla ac uk
Division of Biochemistry & Molecular Biology,
Institute Biomedical & Life Sciences,
Joseph Black Building, University of Glasgow, Glasgow G12 8QQ, UK.
Tel: 0141 330 5167/6459/3089 Fax: 0141 330 8640
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