I presume you tried P32 21 also? Pointless cannot distinguish the
difference..
Refinement at 3A is tricky - you can overfit quite easily and maybe
Rfree is correctly flagging that there are still errors.
Can you see features which need rebuilding?
Eleanor
Emmanuel Prata wrote:
> Dear all,
>
> I have a structure (with 6 dissulphide bridges) at fairly low
> resolution (3A) that I am trying to refine with Refmac. I've used
> phaser with a template with 72% sequence identity. RFZ was 3.4 and TFZ
> 6.6, without clashes, with 80.7% of completeness. Space group was
> p3121 (pointless) and Rmerge 0.12. When i tried refmac, Rfac drop to
> 24%, but Rfree only drop to 41%.
> Can anybody explain this fact?
>
> Thanks in advance,
>
> Emmanuel Prata de Souza
>
>
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