Dear All,
We have solved a structure that has an intersubunit His-His H-bond
(ND1---NE2). The protein most likely undergoes conformational changes
that involve the making and breaking of H-bonds at this interface. In
all the protein structures I have previously studied I don't recall ever
seeing such a bond - His-His ring stacking yes, but never H-bonding. I
was wondering if anyone else had seen this type of interaction and
whether it had any functional significance. The crystals were grown at
pH 5.6.
Many thanks
Dave Lawson
-------------------------------
Dr. David M. Lawson
Biological Chemistry Dept.,
John Innes Centre,
Norwich,
NR4 7UH, UK.
Tel: +44-(0)1603-450725
Fax: +44-(0)1603-450018
Email: [log in to unmask]
Web: http://www.jic.bbsrc.ac.uk/staff/david-lawson/index.htm
|