Hi Emmanuel,
Perhaps a little more information ?
What was the Z-score and LLG in PHASER ? I take it the translation
function solution is fairly well separated from the rest ? Are you sure
the spacegroup is correct ? How complete is the model before refinement
?
Not to be paranoid, but Hexagonal/Trigonal settings support merohedral
twinning...
best,
Iain
-----Original Message-----
From: CCP4 bulletin board [mailto:[log in to unmask]] On Behalf Of
Emmanuel Prata
Sent: Friday, May 11, 2007 10:27 AM
To: [log in to unmask]
Subject: [ccp4bb]. .
Dear all,
I have a structure (with 6 dissulphide bridges) at fairly low
resolution (3A) that I am trying to refine with Refmac. I've used
phaser with a template with 72% sequence identity. RFZ was 3.4 and TFZ
6.6, without clashes, with 80.7% of completeness. Space group was
p3121 (pointless) and Rmerge 0.12. When i tried refmac, Rfac drop to
24%, but Rfree only drop to 41%.
Can anybody explain this fact?
Thanks in advance,
Emmanuel Prata de Souza
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