A 2-years post-doc position is open in Grenoble, France (Institut de 
Recherches en Technologie et Sciences pour le Vivant – iRTSV, CEA), to 
work on:

New fluorescent protein markers to study the molecular and cellular 
dynamics.

Research project:
One of the main goals in cell biology is to characterize molecular 
interaction directly in the intracellular environment. We are interested 
by two techniques, Total Internal Reflection Microscopy (TIRFM, Michelot 
et al., 2006) applied to observe single molecule dynamics and 
Fluorescence Correlation Spectroscopy (FCS or FCCS) that monitors 
protein concentrations and mobilities, protein interaction or 
localization (Bacia et al., 2006). During the past years we developed in 
our laboratory TIRFM to study the dynamics of actin
cytoskeleton in the presence of accessory proteins in vitro (Michelot et 
al., 2006). We would like now to extend this knowledge in a cellular 
environment.To achieve this goal we have to combine different 
expertises. First we have to develop new fluorescent markers with 
improved spectral properties. For this we will use the expertise of 
Dominique Bourgeois's laboratory (LCCP/IBS, partners of this project) 
that studies the link between the structural dynamic and the fluorescent 
properties of proteins such as Green Fluorescent Protein (GFP).

Candidate Profile:
The postdoctoral position in our laboratory (iRTSV/PCV, CEA Grenoble) is 
opened to construct and express new fluorescent markers. These markers 
will be tested first using TIRFM microscopy on the study of the dynamic 
assembly of actin filament or microtubules. We will valid also this new 
fluorescent molecules using FCS or FCCS using the same biological 
system. We will have a strong interest in the structural properties of 
these markers. We will collaborate with Dr. Dominique Bourgeois (IBS, 
Grenoble) for the structural part of this project. The candidate should 
have a strong background in molecular biology, proteins expression and 
purification. Knowledge in crystallography will be very interested to 
develop the structural part of the project and insure the link between 
the different partners of the project.

Contact: [log in to unmask] or [log in to unmask]
-- 
Dominique BOURGEOIS


      /\     Dominique Bourgeois    /\/\
     /  \          LCCP IBS        /\/ /\
  /\/    \         UMR 5075       /  \/  \
/  \     \ 41 Rue Jules Horowitz     \   \
     \     \ 38027 GRENOBLE Cedex 1    \   \
      \     \       FRANCE              \   \

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