I ran the initialiseHSQC macro and I notice this assigns each peak to a
single spin system, with a resonance for the N15 dimension and one for the
1H dimension i.e. not specifically assigning the peak to backbone N15 and 1H
atoms. So is it the case that once I determine which spin system the peak
belongs to, I then have to specifically assign the N15 dimension to the
backbone nitrogen and the H1 dimension to the amide proton?
I understand that this may be because not all peaks in the HSQC will belong
to backbone amides, but the vast majority do so wouldn't it be easier to
assign everything as backbone amides initially and then go in and change the
side-chain peaks afterwards?
cheers,
Kate
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