Hi all.
I am trying to solve a structure with one molecules in the asu through MR.
Using a not good data set at 3.0A and a structure with sequence similarity of
37%, Phaser gave a result of z score 14.7 and LL-gain 220, probably a right
solution.
But when I slightly modified the model I got from Phaser and used it as an
input to run Phaser again, I got another solution overlapped partly with the
first solution. I don't know how it could happen like that. And the Rfree
factor could not go
below 0.43 in the following refinemet. I am wondering what is wrong with it?
thanx in advance
Lei Yin
Lei Yin, Ph.D.
National Laboratory of Biomacromolecules
Institute of Biophysics
Chinese Academy of Sciences
No. 15 Datun Road, Beijing 100101, China
E-mail:[log in to unmask]
Tel: 86-10-64888548
|