Dear all, just a super dummy question: I treated a protein with
trypsin, found the protein being degraded into two well-define
fragments, ran a sizing column to find them co-elute, sent the peak
fraction for mass-spec, got the two masses. Now here is the question -
is there any program readily available for me to roughly identify
these two (around 20K) fragments with the full-length sequence and
these two masses, and of cause, with the fact that I use trypsin to
cut it? I checked a lot of programs listed on Expasy to find them
mostly dealing with smaller peptide length. Any information would be
highly appreciated. Thanks and have a nice weekend, -yong
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