Hi,
Don't forget system losses (friction in the tubing). An empty AKTA prime
typically eats up about 0.25 MPa so your maximum is closer to 0.7MPa loss
on column.
Artem
>
>
> Dear Frank,
> AKTA prime delivers a max. pressure of 1 MPa. The 24 ml SEC columns can
> be run at pressures
> up to 1.5 (S200) or even 3 MPa (superose 12). If your column is in pretty
> good shape, expect to
> reach 0.9 MPa at 0.4 ml/min for S200. So you can use an AKTA prime, but
> ...
> Best
> Clemens
>
>
> At 11:16 PM 2/13/2007, you wrote:
>
> Dear all,
>
> I need to decide between buying an AKTA prime and an AKTA FPLC from GE
> health care. I understand AKTA prime is a low-pressure system, but
> because it is too much cheaper than AKTA FPLC, it is still very
> attractive to me.
>
> I will mainly use it for Nickel columns and gel filtration columns, and I
> am worried about the latter. Is it true that using AKTA prime, you can
> only run the 24 ml Superdex 200 column at 0.1 or 0.2 ml/min?
>
> Could anyone who has used AKTA prime give me some feedbacks? I would
> appreciate it.
>
> Best,
> Frank
>
>
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> Jun.-Prof. Dr. Clemens Steegborn
> Ruhr-University Bochum
> Dept. Physiol. Chemistry, MA 2/141
> Universitaetsstr. 150
> 44801 Bochum, Germany
>
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