Hi all,
Thanks for those who replied so far. I can see that the
solubility issue is not that problematic for the crystallization work
(as Kendall mentioned).
I recall that some people on the board reported in a different
thread that they tried the solid powder in the crystallization drop
and it worked!
The biggest concern is with the planned activity assay! For that,
I think I need to get a clear solution with known concentration of
the compound to be tested.
P.S. Just a correction, I meant we have 10 mg of each compounds.
The compounds received as powder.
At 11:28 AM 2/28/2007, you wrote:
>Dear all,
>
> I have a small library of In-silico screened compounds to test
> for activity and for crystallization trials with our protein of interest.
>
> We only have about 10 mg/ml of each compound. As there is no
> available experimental information about solubility of these
> compounds, I have no choice but to try different solvents.
>
> The first solvent to try will be DMSO (100%) to make the highest
> stock concentration of each compound. My question to those who
> passed through similar experience is:
>
> Assuming some of the compounds turned to be insoluble in DMSO,
> which is possible, how to completely recover the compounds from
> DMSO before trying another solvent.
>
> Will spinning and leaving the tube open over the bench be enough
> to get rid of the solvent or what people usually do in that case?
> What is the recommended solvent to try next?
>
> Is there a standard protocol to follow for the case we have??
>
> thanks in advance for those who are willing to share their experience.
>
> regards,
> Ibrahim
>
>Ibrahim M.Moustafa, Ph.D.
>Pennsylvania State University
>Biochemistry & Molecular Biology Dept.
>201 Althouse Lab.
>University Park, PA16802
>
>Tel (814) 863 8703
>Fax (814) 865 7927
Ibrahim M.Moustafa, Ph.D.
Pennsylvania State University
Biochemistry & Molecular Biology Dept.
201 Althouse Lab.
University Park, PA16802
Tel (814) 863 8703
Fax (814) 865 7927
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