This is really a Q to Kevin Cowtan..
Is it important to have a "proper" mask for DMMULTI? I had always
thought it was self correcting and as long as it was
big enough then the program would update it?
Eleanor
Peter Adrian Meyer wrote:
>> I would be very much afraid that the only way to get the masks
>> properly would be to edit them interactively.
>> I used to do that in the PHASES package by Bill Furey and MapViewX
>> which is still alive and kicking under the BnP flagship.
>>
>> I am pretty sure though that Dr. DVD will be online soon and will
>> point out the correct succession of MAMA/RAVE/O commands ;-)
>>
>>
>
> As far as I know, mapview does section by section 2d editing. Unless
> mapview_x has been updated recently, O is probably less painful for
> interactively editing masks (although my prefered set of mask editing
> commands in O have been declared obsolete).
>
> Pete
>
>
>> A.
>>
>> On Feb 22, 2007, at 1:08, Jianghai Zhu wrote:
>>
>>
>>> Forgot to mention that the NCSs are improper.
>>>
>>>
>>>
>>> On Feb 21, 2007, at 6:22 PM, Jianghai Zhu wrote:
>>>
>>>
>>>> Dear all,
>>>>
>>>> I have two low resolution (3.8 A) MAD data sets from two different
>>>> space groups. There are 4 copies of my molecule in one space
>>>> group and 2 copies in the other. The density-modified maps from
>>>> these data sets are poor, but still allow me to build a crude
>>>> model on them. All the domains should be at the right or close to
>>>> right orientations and positions. I would like to try multi-
>>>> crystal averaging to improve my maps. Since I already have the
>>>> model, I used NCSMASK to make 6 masks for the 6 copies of my
>>>> model. Then I used DMMULTI to perform the multi-crystal
>>>> averaging. The maps came out of DMMULTI showed great
>>>> improvement. Some densities were not seen before showed up
>>>> nicely. However, after I read the manual carefully, I realize
>>>> that DMMULTI would not take care of the mask overlap. So some
>>>> regions in my density map between the NCS-related and symmetry-
>>>> related molecules must be messed up. Could any expert out there
>>>> give me some suggestions about how to make proper NCS masks for
>>>> DMMULTI? Thanks at advance.
>>>>
>>>> Jianghai
>>>>
>>
>
>
> Pete Meyer
> Fu Lab
> BMCB grad student
> Cornell University
>
>
>
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