We have one protein (bacterial antitoxin HigA, 2ICT) that
crystallizes in many conditions (30-40) at 0.3-0.6 mg/ml; the
crystals diffract to 1.63 A.
--
Mark Arbing, PhD
Email: [log in to unmask]
Tel: (212) 854-5236
Fax: (212) 865-8246
Dept. of Biological Sciences
Columbia University
702 Fairchild Center, MC2437
1212 Amsterdam Ave., NY, NY, 10027
Quoting Tassos Papageorgiou <[log in to unmask]>:
> Just to add that in one case we were able to get suitable
> crystals after using a
> protein concentration of as low as 1.5 mg/ml (kapetaniou et al.
> Acta Cryst.
> 2005, F61, 479-481) and microdialysis to slowly remove the excess
> of salt.
> Lower concentrations (0.50-0.75 mg/ml) could also produce
> crystals but they
> were too small.
>
> Tassos Papageorgiou
>
>
>
> Quoting Patrick Shaw Stewart <[log in to unmask]>:
>
> > Ronaldo
> >
> > I have a database of crystallization conditions extracted from
> the
> > PDB. I was able to parse the data to extract the concentration
> of
> > protein in around 900 cases.
> >
> > The lowest 9 protein concentrations were as follows:
> >
> > PDB ID TYPE DATE Protein conc, mg/ml
> > 1EYM ISOMERASE. 07/05/2000 0.75
> > 1ADQ COMPLEX (IMMUNOGLOBULIN/AUTOANTIGEN) 18/02/1997 1
> > 1W5G FOUR HELIX BUNDLE. 06/08/2004 1
> > 1UU4 HYDROLASE. 15/12/2003 1
> > 1W2U HYDROLASE. 08/07/2004 1
> > 1DXP SERINE PROTEASE. 13/01/2000 1
> > 1DY8 SERINE PROTEASE. 18/01/2000 1
> > 1DY9 SERINE PROTEASE. 31/01/2000 1
> > 1DG6 APOPTOSIS. 23/11/1999 1.2
> >
> > The maximum protein conc was 200 mg/ml (1BLF)
> >
> > My database goes up to October 2004. Janet Newman and Tom Peat
> have
> > done this job far more conscientiously and could probably give
> you
> > better and more up-to-date data.
> >
> > Patrick
> >
> >
> > On 2/23/07, Ronaldo Alves Pinto Nagem <[log in to unmask]>
> wrote:
> > >
> > >
> > > Dear all,
> > >
> > > As protein crystallization is the main topic of the day may I
> include
> > > another question?
> > >
> > > What was the minimun protein concentration reported with
> success in
> > > crystallization trials?
> > >
> > > I ask that because the protein I am trying to crystallize is
> much less
> > > soluble than the one mentioned in the lasts emails.
> > >
> > > Thanks
> > >
> > > Ronaldo.
> > >
> >
>
>
> --
> A.C.(Tassos) Papageorgiou, PhD phone: +358 2 333 8012
> (office)
> Senior Scientist, Group leader fax: +358 2 333 8000
> Turku Centre for Biotechnology E-mail:
> [log in to unmask]
> BioCity, Turku URL:
> http://www.btk.utu.fi/~apapageo
> FIN-20521, Finland
>
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