Just to add that in one case we were able to get suitable crystals after using a
protein concentration of as low as 1.5 mg/ml (kapetaniou et al. Acta Cryst.
2005, F61, 479-481) and microdialysis to slowly remove the excess of salt.
Lower concentrations (0.50-0.75 mg/ml) could also produce crystals but they
were too small.
Tassos Papageorgiou
Quoting Patrick Shaw Stewart <[log in to unmask]>:
> Ronaldo
>
> I have a database of crystallization conditions extracted from the
> PDB. I was able to parse the data to extract the concentration of
> protein in around 900 cases.
>
> The lowest 9 protein concentrations were as follows:
>
> PDB ID TYPE DATE Protein conc, mg/ml
> 1EYM ISOMERASE. 07/05/2000 0.75
> 1ADQ COMPLEX (IMMUNOGLOBULIN/AUTOANTIGEN) 18/02/1997 1
> 1W5G FOUR HELIX BUNDLE. 06/08/2004 1
> 1UU4 HYDROLASE. 15/12/2003 1
> 1W2U HYDROLASE. 08/07/2004 1
> 1DXP SERINE PROTEASE. 13/01/2000 1
> 1DY8 SERINE PROTEASE. 18/01/2000 1
> 1DY9 SERINE PROTEASE. 31/01/2000 1
> 1DG6 APOPTOSIS. 23/11/1999 1.2
>
> The maximum protein conc was 200 mg/ml (1BLF)
>
> My database goes up to October 2004. Janet Newman and Tom Peat have
> done this job far more conscientiously and could probably give you
> better and more up-to-date data.
>
> Patrick
>
>
> On 2/23/07, Ronaldo Alves Pinto Nagem <[log in to unmask]> wrote:
> >
> >
> > Dear all,
> >
> > As protein crystallization is the main topic of the day may I include
> > another question?
> >
> > What was the minimun protein concentration reported with success in
> > crystallization trials?
> >
> > I ask that because the protein I am trying to crystallize is much less
> > soluble than the one mentioned in the lasts emails.
> >
> > Thanks
> >
> > Ronaldo.
> >
>
--
A.C.(Tassos) Papageorgiou, PhD phone: +358 2 333 8012 (office)
Senior Scientist, Group leader fax: +358 2 333 8000
Turku Centre for Biotechnology E-mail: [log in to unmask]
BioCity, Turku URL: http://www.btk.utu.fi/~apapageo
FIN-20521, Finland
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