No just common buffer substances: Tris, NaCl.
I think the Nitrogen I used for pressure filtration is solved. It took
a long time, the pressure was high (I think around 5 bar) and I was
stearing it.
Since then it was was in an NMR tube with an stamp on the fluid so that
I noticed every bubble.
Justin
Kris Tesh schrieb:
> Justin,
>
> What is in your solution? Could it be that you are using a salt that at
> your pH equilibrates with the gaseous form? Ammonium Sulfate, Carbonate
> salts and some buffers do this automatically.
>
> Kris
> -----Original Message-----
> From: CCP4 bulletin board [mailto:[log in to unmask]] On Behalf Of Justin
> Schmitz
> Sent: Thursday, February 08, 2007 11:07 AM
> To: [log in to unmask]
> Subject: Re: [ccp4bb] Good NMR Board
>
> Thank you for all the replies.
>
> @ Kris
> It is not the problem of single gas bubbles, but of solved gas. I
> prepared the sample in the middle of December and it still degases that
> means bubbles appear. They can be removed but after a few days new ones
> are coming up. I'm doing NMR and the shimming always gets worse if this
> appears. So it is difficult to get a good 3D dataset if in the middle of
> the measurement the sensitivity breaks down.
> I'm searching for a way to simple degas the sample. I put it in normal
> vacuum in tube, but that doesn't work good because of the small surface.
> Enlarging the surface would create the risk of abstracting too much
> water so that the concentration rises to fast and the Protein aggregates.
> Is there another way?
> Tomorrow I will try there syringe method.
>
> Justin
>
>
>
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