Ronaldo
I have a database of crystallization conditions extracted from the
PDB. I was able to parse the data to extract the concentration of
protein in around 900 cases.
The lowest 9 protein concentrations were as follows:
PDB ID TYPE DATE Protein conc, mg/ml
1EYM ISOMERASE. 07/05/2000 0.75
1ADQ COMPLEX (IMMUNOGLOBULIN/AUTOANTIGEN) 18/02/1997 1
1W5G FOUR HELIX BUNDLE. 06/08/2004 1
1UU4 HYDROLASE. 15/12/2003 1
1W2U HYDROLASE. 08/07/2004 1
1DXP SERINE PROTEASE. 13/01/2000 1
1DY8 SERINE PROTEASE. 18/01/2000 1
1DY9 SERINE PROTEASE. 31/01/2000 1
1DG6 APOPTOSIS. 23/11/1999 1.2
The maximum protein conc was 200 mg/ml (1BLF)
My database goes up to October 2004. Janet Newman and Tom Peat have
done this job far more conscientiously and could probably give you
better and more up-to-date data.
Patrick
On 2/23/07, Ronaldo Alves Pinto Nagem <[log in to unmask]> wrote:
>
>
> Dear all,
>
> As protein crystallization is the main topic of the day may I include
> another question?
>
> What was the minimun protein concentration reported with success in
> crystallization trials?
>
> I ask that because the protein I am trying to crystallize is much less
> soluble than the one mentioned in the lasts emails.
>
> Thanks
>
> Ronaldo.
>
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