Hi,
I would suggest that you may harvest the crystals at different time, and
then mount them and test at room temperture.
Our lab has the experience that large crystals always have lower resolution
than medium size(0.1-0.2mm). So try and good luck.
deliang
----- Original Message -----
From: "Juergen Bosch" <[log in to unmask]>
To: <[log in to unmask]>
Sent: Wednesday, January 24, 2007 12:29 PM
Subject: Re: [ccp4bb] Fancy crystal, poor diffraction
> Hi Tiancen,
>
> nobody so far has suggested to mount the smallest crystals, 0.4x0.4x0.3
> seems to be pretty large, this also means that the freezing procedure is
> longer. Then the other question is do you freeze in the Cryostream or by
> plunging into LN2, I'd prefer the latter technique.
>
> Good luck,
>
> Juergen
>
>
>
> --
> Jščrgen Bosch
> University of Washington
> Dept. of Biochemistry, K-426
> 1705 NE Pacific Street
> Seattle, WA 98195
> Box 357742
> Phone: +1-206-616-4510
> FAX: +1-206-685-7002
>
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