> We have crystallized a 21KD protein with 2 disulfide bonds grown for one
> month in 0.1M tri-sodium citrate pH 5.6, 0.5M (NH4)2SO4 and 1M Li2SO4. The
> crystals look big (~0.4mm x 0.4mm x 0.3mm) and pretty (sharp edge, clean
> surface) but diffracted to only 4A in-house. The spots are quite strong
> and isotropic at low resolution but decay sharply beyond 5-6A. The crystal
Have you tried DTT (or another way of keeping things in a reducing
environment)? This might help keep your disulfides (and hopefully
protein) happy.
Pete
Pete Meyer
Fu Lab
BMCB grad student
Cornell University
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