Thanks!
Phoebe
At 05:56 PM 1/22/2007, you wrote:
>Hi Pheobe,
>I remember an interesting paper that described how a structure revealed a
>surprising role the buffer was playing in inhibition:
>
>The 1.20 A resolution crystal structure of the aminopeptidase from
>Aeromonas proteolytica complexed with tris: a tale of buffer inhibition.
>
> * Desmarais WT,
> * Bienvenue DL,
> * Bzymek KP,
> * Holz RC,
> * Petsko GA,
> * Ringe D.
>
>
>http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Abstract&list_uids=12176384
>
>-----Original Message-----
>From: CCP4 bulletin board on behalf of [log in to unmask]
>Sent: Mon 1/22/2007 3:29 PM
>To: [log in to unmask]
>Subject: : misbound ligand examples?
>
>A biochemist friend asked for examples of cases were a protein was
>co-crystallized with or soaked in a ligand that bound in the wrong place -
>say, because the ligand used wasn't quite the right one or because other
>important ligands were absent.
>I'm sure such examples are out there, especially when soaks were done at
>high concentrations, but I'm having trouble thinking of concrete examples.
>Help?
>thanks,
>Phoebe Rice
>
>
>---------------------------------------------------------------------------------------------------------------------------
>Phoebe A. Rice
>Assoc. Prof., Dept. of Biochemistry & Molecular Biology
>The University of Chicago
>phone 773 834 1723
>fax 773 702 0439
>http://bmb.bsd.uchicago.edu/index.html
>http://www.nasa.gov/mission_pages/cassini/multimedia/pia06064.html
---------------------------------------------------------------------------------------------------------------------------
Phoebe A. Rice
Assoc. Prof., Dept. of Biochemistry & Molecular Biology
The University of Chicago
phone 773 834 1723
fax 773 702 0439
http://bmb.bsd.uchicago.edu/index.html
http://www.nasa.gov/mission_pages/cassini/multimedia/pia06064.html
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