> I'm using Paul Driscoll's ubiquitin data to learn about Analysis (after
> working through the tutorial) and managed to peak pick, set spin systems
> for the HSQC and then propagate them to the HNCACB and HN(COCA)CB. The
> sequence has also been loaded and the molecule generated from it. So far ,
> so good.
> Now I want to start assigning, but when I open the 'Link Sequential Spin
> Systems' panel and follow the instructions from the tutorial about match &
> query windows the 'Spin Systems' table remains empty. How do I get my spin
> systems into the table so I can start having fun with the assigments? I
> guess I missed something obvious...
The spin systems that appear are those that are linked to the 'root'
dimensions of the peaks of the spectra visible in the query window.
Given that you say the root resonance assignments exist on both spectra
then we can hopefully rule out missing spin systems. Firstly check for
error messages on the command line. Then I suggest you check that the
query spectrum is actually toggled to "on" in the query window and that
the peak list that carries the spin system assignments in the query
spectrum is set to the active peak list: You can see if you have multiple
peak lists and set which is active in the Current Peak Lists popup.
If nothing presents itself, then sending me a screen-shot image may help.
Thanks,
Tim
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Dr Tim Stevens Email: [log in to unmask]
Department of Biochemistry [log in to unmask]
University of Cambridge Phone: +44 1223 766018 (office)
80 Tennis Court Road +44 7816 338275 (mobile)
Old Addenbrooke's Site +44 1223 364613 (home)
Cambridge CB2 1GA WWWeb: http://www.bio.cam.ac.uk/~tjs23
United Kingdom http://www.pantonia.co.uk
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