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But then I guess it is not a 'full 6D search' anymore (but a lot more
dimensions), because you want to position all the proteins, right?
Serge.
Le 23 janv. 07 à 04:14, Bart Hazes a écrit :
> Hi Filip,
>
> You're right and the same applies if the MR is difficult because of
> differing relative domain orientations in otherwise closely related
> to proteins. As mentioned, my remark was aimed at distantly related
> search models.
>
> Bart
>
> Filip Van Petegem wrote:
>
>> But that isn't necessarily the case if the search is hard because
>> your
>> search models individually constitute only a small part of the
>> asymmetric unit. Say that 80% of the AU consists of multiple
>> different proteins with known structure; the phase information would
>> be very high if you find the solutions.
>>
>> Filip
>>
>> On 1/22/07, Bart Hazes <[log in to unmask]> wrote:
>>
>>> I'd like to add that the value of a molecular replacement
>>> solution tends
>>> to be inversely correlated with the effort needed to find the
>>> solution.
>>> In other words, the harder you have to work to find the MR
>>> solution the
>>> less informative the phase information you tend to get. When you
>>> have
>>> very high resolution and/or NCS you may still be able to solve the
>>> structure. However, in cases were the search model is only distantly
>>> related to the protein of interest and Phaser can't find the
>>> solution,
>>> the solution may not be worth finding and you're better of
>>> focussing on
>>> getting experimental phases.
>>>
>>> Bart
>>>
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