>> Have you tried DTT (or another way of keeping things in a reducing
>> environment)? This might help keep your disulfides (and hopefully
>> protein) happy.
>
>
> Surely the addition of a _reducing_ agent would _reduce_ your disulphides
> -
> i.e. break them.
Whoops...sorry, I guess I've got zinc sites on the brain.
Thanks for the correction,
Pete
Pete Meyer
Fu Lab
BMCB grad student
Cornell University
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