Please make sure the title of posts to this list matches the contents - I
might have missed this query thinking it was a Format Conveter issue...
> I have an experiment which is an hncoca and I have set the
> experiment type H [N[co[CA]]]. However it only seems to have one
> ExpTransfer between 1H and 13C there is no H N ExpTransfer
Hmm. There should not be an ExpTransfer between the 1H and 13C as the
transfer to the 13Ca comes from the unmeasured carbonyl. It is possible
that the mapping between the ExpDimRefs and the RefExpDimRefs needs
looking at when setting the experiment type. This is an odd one because
with different isotopes for each axis there should be no ambiguity with
setting this mapping (e.g. unlike HCCH).
> 2. if I change the type of the experiment to an HNCO I get two
> ExpTransfers again
Here there really should be two between 1H & 15N and between 15N & 13C.
It would be worth checking that both transfers for HNCO are sensible even
if there are two in total. If the transfers are between the wrong dims I
would suggest that when the spectrum is loaded, the wrong isotope type is
coming through/getting set for the dim numbers.
> 3. if I add a new spectrum and set the type to hncoca again I only get
> one ExpTransfer
When I use my HNcoCA to test I get one transfer between the 1H and 15N as
expected, so I'm a bit baffled.
Check that the isotopes match the dims and then the refExpDims. If there
is still no joy maybe you could send the spectrum data or param file.
T.
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Dr Tim Stevens Email: [log in to unmask]
Department of Biochemistry [log in to unmask]
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