-----BEGIN PGP SIGNED MESSAGE-----
Hash: SHA1
Description of the problem:
I have a set of triple resonance experiments and a 15N-HSQC. I
initially picked the peaks from all the spectra anonymously. Given
the set of typical triple resonance spectra, I was able to connect
the peaks from the same residue and its preceding residue to their
corresponding amide peak (NH) in the 15N-HSQC, i.e., establish i and
i-1 connectivity of the anonymously numbered spin systems.
Eg., HNCA: {5}N[10],{5}N[11],{5}CA[200] (added to the spin system
using 'R:' menu); {5}N[10],{5}N[11],{6}CA[203] (added to the
sequential spin system using 'R:' menu)
HNcoCA: {5}N[10],{5}N[11],{6}CA[203]
Please see below, 'steps to reproduce.'
Version-Release number of selected component:
1.0.9 (may have been present in 1.0.8)
How reproducible:
Always
Steps to Reproduce:
1. Assign a set of spectra as described above in the 'description of
the problem.'
2. Now assign residue specific information and convert the annonymous
spin system created in the previous step, eg., {5}N and {5}H to a
(named and numbered) residue in the sequence of your molecule, say, MS1.
Actual Results: T
In the example above, spin system {5} and its corresponding intra-
residual CA, {5}CA, gets the residue specific assignment (name and
number) from MS1, eg., {5}CA becomes 4TyrCA (an atom of, say, residue
i). However, despite the presence of inter-residual connectivity
(say, i-1) information from the above steps, anonymous spin system {6}
CA remains anonymous.
Expected Results:
You expect the inter-residual peak to get the corresponding
assignment automatically. However, it does not. Exceptions are
spectra like HNCA which have both i and i-1 information, where it
seems to work fine. It remains anonymous in spectra that give only
i-1 information like HNcoCA, HNCO.
Additional info:
NA
-----BEGIN PGP SIGNATURE-----
Version: GnuPG v1.4.1 (Darwin)
iD4DBQFD8xSEm7hHUYJtaG4RAqlcAJdlklAs/GyNzI5xJdfWaj9PnSGtAJ48fz2C
0c1nfIfr8EbXiW1qdBaYYw==
=7zIM
-----END PGP SIGNATURE-----
|