> when I tried to do the same thing with the HCCONH spectrum (widow
> mapping: 1H 1H 15N, spectrum dimensions: 1H 15N 1H), "assign root
> resonances" function wouldn't assign the picked HCCONH peaks from the
> corresponded HSQC peaks no matter how I adjusted the tolerances on HN
> dimensions. Would that be because analysis is confused by two proton
> dimensions and not able to tell which dimension the resonances should go
> to?
Theoretically the two 1H dims are distinguished by the reference experiment
that your HCCONH experiment is linked to, so maybe check that this is set
OK (Experiment::Experiment Types) - looking especially at lower Reference
Dimensions table for the experiment: here you can swap over the mapping of
experimental dimension to reference dimension (which ultimately contains
the info about which dims are bonded).
The system works with my own 1H,1H,15N experiments. If the reference info
all seems OK I will investigate further.
T.
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Dr Tim Stevens Email: [log in to unmask]
Department of Biochemistry [log in to unmask]
University of Cambridge Phone: +44 1223 766022 (office)
80 Tennis Court Road +44 7816 338275 (mobile)
Old Addenbrooke's Site +44 1223 364613 (home)
Cambridge CB2 1GA WWWeb: http://www.bio.cam.ac.uk/~tjs23
United Kingdom http://www.pantonia.co.uk
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