I'm not positive I understand the question (so someone with more NMR sense
might have to comment) but it looks like you have unusual 15N ppm values
outside the default "universe" as defined by Analysis. You can change
that by going into Other --> Axes and Panels and in the middle table you
can change the size of the "universe" for each isotope type in the sixth
("Region") column. (The default for 15N is 80, 135.)
Wayne
On Fri, 27 Jan 2006, Paul Driscoll wrote:
> I am monitoring changs to a 15N spectrum where I follow both His ring NH's
> at ca. 200 ppm and Arg/Lys side chain NH at ca. 70 ppm. I do this by
> recording two separate ('unfolded') spectra with different 15N offsets, so
> as not to get off resonance errors. When I load these into analysis I have
> some issues:
>
> To make the spectra look sensible I have to adjust the aspect ratio (to
> e.g. 4), but then the scroll bar becomes too big to move it up and down
> the 15N axis. Also I seem to lose the top and bottom of the
> combined spectra. I
> thought maybe 'slice range' would be able to adjust for an extra wide
> sweepwidth but I am not very clear what this parameter is, or what units
> it is in.
>
> Am I testing the limits by having an unusual requirement or am I being
> dumb?
>
> Paul
>
>
> --
>
> Paul C. Driscoll
> Professor of Structural Biology
> ---------------------------------------------------------------------
> Prof. Paul C. Driscoll |Office (answer)phone: (44)-20 7679 7035
> Dept. Biochem. & Mol. Biol. | Department fax: (44)-20 7679 7193
> University College London | [log in to unmask]
> Gower Street, London WC1E 6BT| http://www.biochem.ucl.ac.uk/~driscoll
>
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