I am monitoring changs to a 15N spectrum where I follow both His ring NH's
at ca. 200 ppm and Arg/Lys side chain NH at ca. 70 ppm. I do this by
recording two separate ('unfolded') spectra with different 15N offsets, so
as not to get off resonance errors. When I load these into analysis I have
some issues:
To make the spectra look sensible I have to adjust the aspect ratio (to
e.g. 4), but then the scroll bar becomes too big to move it up and down
the 15N axis. Also I seem to lose the top and bottom of the
combined spectra. I
thought maybe 'slice range' would be able to adjust for an extra wide
sweepwidth but I am not very clear what this parameter is, or what units
it is in.
Am I testing the limits by having an unusual requirement or am I being
dumb?
Paul
--
Paul C. Driscoll
Professor of Structural Biology
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