Dear All,
I recently exported all my resonance (complete list from 15N-HSQC and
triple resonance experiments). When I processed the data and grouped
them for each HSQC peak, I find 50% of them have been duplicated
exactly for all the experiments. Just the spin system number changes.
It is mostly an anonymous assignment (connections established for i
and i-1) except for about a tenth of the sequence. The duplications
range from exact duplication to many copies, but all with unique spin
system numbers
However, nothing seems to logically explain this to me. ANy ideas or
suggestions or explanations, etc. would be very welcome!
When I initially started on this project, I initialised the HSQC
using the in-built macro. It selected a lot of peaks which I deleted
because they were not appropriate. I didn't delete the resonance.
Could this cause analysis to link the resonance to appropriate and
corresponding experiments and causing it to appear like duplications?
WHy does these automatic connections occur, if I am right?
Many thanks.
Best,
Murali.
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