Hi Di Jiang,
Did you already solve problems with fertilization of Styela?
I used to obtain sperm by dissection of gonads. The sperm is usually immature
and should be activated by high pH. Did you have a look at sperm under
the microscope? Were sperm swimming? If not, an easy method to activate
sperm is to add 150 microliters of 0.1 N NaOH to 10 ml of seawater
containing diluted sperm.
Good luck.
Shigeki Fujiwara
Department of Biology
Faculty of Science
Kochi University
Kochi 780-8520
Japan
phone: 81-88-844-8317
fax: 81-88-844-8356
e-mail: [log in to unmask]
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