Dear all,
Please note this RSS talk next thursday:
RSS HIGHLANDS LOCAL GROUP MEETING
DATE: Thursday, 4/3/2004
TIME: 6 pm
PLACE: Meston Building, University of Aberdeen, Room 302
TEA: 5.45 pm
TITLE:
Design, Normalization and Quality Control for Two Colour cDNA Microarray
Experiments.
SPEAKER:
David Baird
Abstract:
This talk will look at statistical issues in experiments using two
colour cDNA microarrays. The design of these microarray experiments is
equivalent to using an incomplete block design with blocks of size 2.
However, the additional requirement of removing any dye bias effects
adds another nuisance term into the analysis which must be estimated
efficiently by the design chosen. Further, with experiments using
multiple animals per treatment, between animals effects may want to be
estimated. Designs which take into account these issues will be
presented. Once the results for each spot on the slides have been
obtained, the results should be normalized to remove further nuisance
effects induced by the printing, hybridization, scanning and image
analysis processes. For this normalization, a REML analysis which takes
into account spatial, dye intensity and carryover effects between
adjacent spots is presented. Subsequent to this analysis, issues such as
non-constant variance of the estimated effects must be considered and
allowed for. Also the quality of individual slides can be examined, once
the adjusted effects have been estimated. Finally, the results must be
summarized in a manner which provides information useful in selecting
interesting items from the library of cDNA printed onto the slide and
our approaches to this will be presented.
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Everybody welcome (including non-RSS-members and non-statisticians)
Regards,
Claus Mayer,
Secretary, Highlands Local Group
(http://www.maths.abdn.ac.uk/maths/department/rss/highlands.html)
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Claus-D. Mayer | http://www.bioss.ac.uk
Biomathematics & Statistics Scotland | email: [log in to unmask]
Rowett Research Institute | Telephone: +44 (0) 1224 716652
Aberdeen AB21 9SB, Scotland, UK. | Fax: +44 (0) 1224 716608
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