Listmembers,
I request some help with implementation of Youden's one-sample regression to
identify the Youden blank in an analytical test method.
A test sample is reacted in solution to produce a byproduct. A reagent is
then added to the sample solution to react with the byproduct and produce a
colored byproduct. The colored solution is read in a spectrophotometer. Signal
strength at the appropriate wavelength is read and related to concentration by
a calibration or standard line prepared earlier. The standard line is
prepared from authentic samples of pure colored byproduct in the same solvent system
but at different strengths or concentrations. Signal strength vs.
concentration is linear over the range of interest, meeting the lack-of-fit test and
unpatterned residual distribution requirements.
How would I determine the Youden blank? Do I prepare test sample solutions
at different concentrations, react them, add reagent and produce the colored
byproduct, then read the signal strengths and regress signal strength vs.
concentration and take the y-intercept as the Youden blank? What is the
significance of this blank? How is it to be used?
To anyone who is familiar with Youden's one-sample regression procedure,
interpretation of the y-intercept (Youden blank), and is willing to spend some
email time with me, I would be most grateful.
Regards,
Stan Alekman
Let charm be thy spear, wit thine armor.
Stanley L. Alekman Ph.D.
Pharmaceutical Consultant
S. L. Alekman Associates, Inc.
Inverness, Illinois
home office: 847-358-6975
fax: 847-358-7417
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