Hi Mike. In the field, to test for death in Styela, I simply press on
them gently to see if they'll squirt. If they squirt at all, I consider
them alive, and if not, then dead. Why are you using acetic acid to
cause death? Mary
Mike Scruton wrote:
> Hi,
> I am a masters student doing some work on the invase Styela clava (clubbed
> tunicate). My work is dealing with the pathophysiology of death in this
> species, in particular, the effects of acetic acid on the tunic. If anyone
> is aware of any articles related to the tunic of Styela, or related
> treatments trials, your insight would be greatly appeciated. Also, I am
> having a hard time determining when the tunicates are actually dead. I
> have tried using ECG to determine this, however this was unsuccessful. I
> worry that the tunicates are dead immediately after treatment, although I
> can't tell for sure until they rot a week or so later. If anyone is aware
> of any method to determine death in tunicates, that would also be extremely
> helpful for my project. Thanks a lot.
>
> Mike
--
Mary R. Carman
Research Associate II
Geology & Geophysics
Woods Hole Oceanographic Institution
Woods Hole, MA 02543
Phone 508-289-2987
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