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TARDIGRADA Home

TARDIGRADA  2000

TARDIGRADA 2000

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Subject:

Mounting Media

From:

"Clark Beasley" <[log in to unmask]>

Reply-To:

[log in to unmask]

Date:

Mon, 11 Sep 2000 16:33:58 -0500

Content-Type:

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text/plain (30 lines)

Thanks, Nigel, for including directions for your media.

I mostly have used variations of Hoyer's -- there are many.  The one I have
used in the past is in the monograph (Faure's).  More recently, I have used
one from Krantz's "A Manual of Acarology".  Of course, like everyone else,
he simply calls it Hoyer's medium.  The recipe for it is:

	Distilled water		50 grams
	Gum arabic (crystals)	30 grams
	Chloral hydrate	           200 grams
	Glycercine		20 grams

His directions are, essentially, add ingredients in order, dissolving as you
go.  Filter before use.
It works well, but I am always looking for something better (i.e., provides
better contrast under phase).

I used one formula of polyvinyl lactophenol medium years ago and quit using
it.  I think it was because a few slides would crystallize.

Clark W. Beasley
Department of Biology
McMurry University
Abilene, TX  79697
USA



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